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  产品展示 > 核酸纯化 > RNA提取
RN38-EASYspin Plus 植物RNA快速提取试剂盒   点击次数:18554次

货号#
规格
价格
RN3802
50
1300.00 

产品介绍:

     本公司独家推出EASYspin无苯酚、氯仿RNA快速提取技术基础上,又独家研发成功基因组DNA清除柱技术可以有效清除gDNA残留,得到的RNA一般不需要DNase消化,可用于反转录PCR、荧光定量PCR等实验。独特的裂解液迅速裂解细胞和灭活细胞RNA酶,植物RNA助提剂PLANTaid帮助结合多糖多酚并通过离心去除,然后裂解混合物用乙醇调节RNA结合吸附到基因组DNA清除柱,基因组DNA清除柱子同时吸附清除残留的DNA, 然后RNA被选择性洗脱滤过。滤过的RNA用乙醇调节结合条件后,RNA在高离序盐状态下选择性吸附于离心柱内硅基质膜, 再通过一系列快速的漂洗-离心的步骤, 去蛋白液和漂洗液将细胞代谢物,蛋白等杂质去除, 最后低盐的RNase free H20将纯净RNA从硅基质膜上洗脱。

产品特点:

1.完全不使用有毒的苯酚,氯仿等试剂,也不需要乙醇沉淀等步骤。
2.简捷,单个样品操作一般可在25分钟内完成,世界上最简单快速的试剂盒。
3.独有的植物RNA助提剂可以有效结合多糖多酚,提高清除效果。
4.独家研发成功基因组DNA清除柱技术可以有效清除gDNA残留,得到的RNA一般不需要DNase消化,可用于反转录PCR、荧光定量PCR等实验。
5.适应性极其广泛,可以提取包括棉花、松针、冬青树叶、葡萄叶片、等100多种国内外试剂盒提取失败的样品。详细样品列表请参考公司主页产品介绍。
6.多次柱漂洗确保高纯度,OD260/OD280典型的比值达1.9~2.2,基本无DNA残留,可用于RT-PCR,Northern-blot和各种实验。
使用EASYspin/EASYspin Plus试剂盒已经有大量文章发表,请联系我们索取发表的文章和包括北京林业大学,北京农学院,中国农业大学,农林科学院果树研究所,林业所,中国农科院等大学和研究院所大量使用案例。qq:328153626maozhang2000@hotmail.com  13691030050

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5.       1 EXPRESSION, DIVERGENCE AND EVOLUTION OF THE CALEOSIN GENE FAMILY IN BRASSICA RAPA. Arch. Biol. Sci., Belgrade, 65 (3), 863-876, 2013 DOI:10.2298/ABS1303863H

6.     番茄叶:Effect of Low Temperature Stress on the Expression of ProDH Gene and the Activities of the Proline Dehydrogenase in Leaves of Tomato Seedling. Chinese Agricultural Science  Bulletin 2012,28(10):132-135

7.     栀子叶:Isolation of High Quality Total RNA from Gardenia jasminoides Eills.Chinese Agricultural    Science Bulletin.2012, 28(27):194-198

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13. 大豆:RNA-seq Analysis Reveals Ethylene-Mediated Reproductive Organ Development and Abscission in Soybean(Glycine max L. Merr.). Plant Mol Biol Rep, 2012, published online: 4 Dec, 2012

14. 大豆1Construction of ethylene regulatory network based on the phytohormones related gene transcriptome profiling and prediction of transcription factor activities in soybean. Acta Physiol Plant, 2012, published online: 12 Dec, 2012

15. 红花玉兰:Expression Analysis of MAwuAG in Different Organs and Developmental Stages of Magnolia wufengensis. Chinese Bulletin of Botany, 2013, 48 (2): 1–5

16. 毛桃:Cloning and Phylogeny Analysis of PpAP2 Floral Homologous Genes in Peach. Chinese Agricultural Science Bulletin, 2013, 29(7): 99-104

17. 五倍子:Cloning and characterisation of a phenylalanine ammonia-lyase gene from Rhus chinensis. Plant Cell Rep, 2013, published online15 March, 2013

18. :五倍子1Cloning, characterization and expression of chalcone synthase from medicinal plant Rhus chinensis.J. Plant Biochem. Biotechnol. DOI 10.1007/s13562-013-0231-9

19. 青杄 cDNA Cloning and Bioinformatic Analysis of the sPPa1 Gene form Picea wilsonii. Plant Science Journal, 2012, 30(40): 394-401

20. 青杄 1cDNA Cloning and Bioinformatic Analysis of PsbO Gene from Picea wilsonii.Life Science Research, 2012, 16(3): 201-206

21. 青杄 2Cloning and Tissue Expression Analysis of PwPSAF in Picea wilsonii. SCIENTIA SILVAE SINICAE. Vol. 49No. 10, Oct. 2013.

22. 洋葱:Molecular Cloning and Transcriptional Analysis of the Putative AGAMOUS Homolog AcAG in Onion (Allium cepa. Plant Mol Biol Rep, DOI 10.1007/s11105-013-0607-y

23. 木瓜:XsFAD2 gene encodes the enzyme responsible for the high linoleic acid content in oil accumulated in Xanthoceras sorbifolia seeds. JOURNAL ARTICLE. 2013-6-17.

24. 木瓜1Two novel diacylglycerol acyltransferase genes from Xanthoceras 2 sorbifolia are responsible for its seed oil content. GENE-38688; No. of pages: 9; 4C:

25. 柑橘:Efficient auto-excision of a selectable marker gene from transgenic citrus by combining the Cre/loxP system and ipt selection. Plant Cell Rep, DOI 10.1007/s00299-013-1470-x

26. 柑橘1Expression Analysis of Three Phloem-specific Promoters in Transgenic Poncirus trifoliata. Acta Horticulturae Sinica. 2014, 41(1): 18.

27. 柑橘2 Activation of three pathogen-inducible promoters in transgenic citrus (Citrus sinensis Osbeck) after Xanthomonas axonopodis pv. citri infection and wounding. Plant Cell Tiss Organ Cult. DOI 10.1007/s11240-013-0423-y.

28. 茶梅花瓣:Comparison and Analysis of Methods of Extracting Total RNA from Petals of Camellia sasanqua. Chinese Agricultural Science Bulletin.2013,29(28):129-133.

29. 栀子:Isolation of High Quality Total RNA fromGardenia jasminoides Eills. Chinese Agricultural Science Bulletin. 2012, 28(27):194-198

30. 丹参:Genome-wide analysis and molecular dissection of the SPL gene family in Salvia miltiorrhiza. 2014 Jan;56(1):38-50. doi: 10.1111/jipb.12111. Epub 2013 Nov 20.

31. 牡丹:Transcriptome Comparison Reveals Key Candidate Genes Responsible for the Unusual Reblooming Trait in Tree Peonies. Genes Responsible for Reblooming in Tree Peonies. November 2013 | Volume 8 | Issue 11 | e79996

32. 东南景天:Role of sulfur assimilation pathway in cadmium hyperaccumulation by Sedum alfredii Hance. Ecotoxicology and Environmental Safety. Volume 100, February 2014, Pages 159–165.

33. 山苍子:Identification of appropriate reference genes for normalizing transcript expression by quantitative realtime PCR in Litsea cubeba. TECHNICAL NOTE. Mol Genet Genomics (2013) 288:727–737, DOI 10.1007/s00438-013-0785-1

34. 木本植物:Heterologous gene silencing induced by tobacco rattle virus (TRV) is efficient for pursuing functional genomics studies in woody plants. ORIGINAL PAPER. Plant Cell Tiss Organ Cult, DOI 10.1007/s11240-013-0393-0

35. 棉花:Analysis of sea-island cotton and upland cotton in response to Verticillium dahliae infection by RNA sequencing. Sun et al. BMC Genomics 2013, 14:852 /1471-2164/14/852.

36. 桃子:Biochemical changes and defence responses during the development of peach gummosis caused by Lasiodiplodia theobromae. Eur J Plant Pathol (2014) 138:195–207, DOI 10.1007/s10658-013-0322-4.

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39. 海藻:A rapid and sensitive method for field detection of Prorocentrum donghaiense using reverse transcription-coupled loop-mediated isothermal amplification. Harmful Algae 29 (2013) 31–39.

40. 油茶:Establish a cDNA-AFLP Technology System in Camellia oleifera. Molecular Plant Breeding, 2013, Vol.11, No.5, 611-616.

41. 亚洲百合:Transcriptomic analysis of Asiatic lily in the process of vernalization via RNA-seq. Mol Biol Rep. DOI 10.1007/s11033-014-3250-2.

42. 毛泡桐:Dynamic expression of novel and conserved microRNAs and their targets in diploid and tetraploid of Paulownia tomentosa. Biochimie xxx (2014) 1e10.

43. 人参:Cloning and Sequence Analysis Squalene Epoxidase Gene in Panax gin-seng. Journal of Jilin Agricultural University 2014, 36(2): 149-152,17

44. 雪莲:Cloning and Sequence Analysis of rbcs Gene from Sasussured involucrdta Kar. et Kir. Chinese Agricultural Science Bulletin 2014, 30(15): 261-267

45. 柑橘3Secreted Expression of Cecropin B Gene Enhances Resistance to Xanthomonas axonopodis pv. citri in Transgenic Citrus sinensisTarocco Acta Horticulturae Sinica 2014, 41(3): 417428 http: // www. ahs. ac. cn

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其它公司多糖多酚植物RNA提取试剂盒失败原因和解决方案

很多植物RNA的样品由于含有大量的多糖、多酚、代谢产物、色素等成分,造成RNA提取过程中氧化、褐化、降解、由于植物品种的多样性造成情况更加复杂。手工的CTAB类的方法提取因为时间太长,太繁琐,手工方法不在讨论之列。一直以来没有一款好的试剂盒包括qiagenpromega等进口试剂盒也无法满足科研工作者对植物RNA提取的要求。

下面我们来分析一下植物RNA为什么不能提取成功的原因:

市面上最常见的RNA提取试剂盒无非是两种:第一种:TRIzol改良类方法(包括溶液型的和离心柱型的)、第二种:直接裂解过柱子的方法(离心柱)

第一种试剂盒失败的原因1RNA市面上面最流行的方法就是Trizol,或者Triol改良,或者Trizol加离心柱一类的改良方法。trizol也就是异硫氰酸胍/苯酚/氯仿原理一步法的方法最适合的对象是动物源性的组织细胞,针对普通多糖多酚低的植物性的材料,TRIzol类原理产品也可以提取。但是多糖、多酚、次级代谢产物丰富的情况下,trizol类方法无法防止多糖多酚对于RNA/DNA分相的干扰,要么残留大量DNA,要么残留大量多糖、多酚或者次级代谢产物,氧化破坏RNA,或者残留这些多糖多酚,色素代谢产物等抑制下游的反转录等反应。限于技术水平的限制,市面上绝大多数的国产厂家是使用trizol的方法进行改良,无论是不是加了离心柱。但是实践证明,改良不能从根本上解决问题。判断是否试剂盒使用这种改良的方法非常简单:是否裂解液含有苯酚的味道和使用氯仿,如果使用到了氯仿就是TRIzol方法的改良。

第二种试剂盒失败的原因:直接裂解过柱子的方法是目前最先进的方法,但是也是技术含量最高的方法。这个方法采用裂解液(不含苯酚,氯仿)直接裂解,RNA/DNA同时过柱子,然后在柱子上面直接分离RNA/DNA,所以,这种方法的优点第一在于,避免了使用trizol在多糖多酚下不能成功分离RNA/DNA的弊端、第二在于,不使用有毒的苯酚氯仿。但是正是因为其技术先进,所以难度很高,国内厂家包括进口公司有两个技术难点一直没有突破。第一,裂解液的成分必须针对去除多糖多酚进行研发添加去多糖多酚,代谢产物成分。否则会同样碰到多糖多酚干扰提取的问题。第二、和trizol原理不同,直接过柱法DNA/RNA同时加到吸附柱上去。如何去除DNA是第一个难点。否则会残留大量DNA。两个技术难点的没有掌握导致了国内公司包括的第二种试剂盒失败。国外公司因为没有掌握第一难点,裂解液里面没有去除多糖多酚成分,所以包括qiagen的盒子也常常不能成功提取植物RNA样品。

本人领衔和研发团队配合经过3年的不断研发改良,针对多糖多酚植物的特点,和这两种试剂盒失败的原因,开发出了EASYspin植物RNA快速提取试剂盒,第一采用直接过柱子方法,彻底抛弃了TRIZOL苯酚,氯仿原理方法,使用无毒原料,并且添加了有自主知识产权的去除多糖多酚成分解决了多糖多酚和代谢产物对于RNA的破坏和干扰分离。第二突破了直接过柱子的方法DNA去除的技术难点,解决了DNA残留过多问题。经过实践过程中,几十种国内试剂盒提取失败和进口试剂盒提取失败的例子,使用我们开发的试剂盒提取,除了一例因为离心柱子堵塞导致失败外,全部成功。而且,20分钟提取步骤非常简单,非常快速,无毒苯酚氯仿。全部提取成功的RNA可以成功完成下游反应试验。

部分成功样品:

植物:棉花、海棠、黑加仑、烟草、拟南芥、虎杖、大豆、草莓、冬青、月季花雌蕊、蔷薇、沙棘、冬枣、芦荟、仙人掌、报春花、水稻、玉米、唐菖蒲、樱桃、白玉兰、毛白杨、樱花、葡萄、百合花、百合叶子雌蕊雄蕊、紫菜、绿藻、香蕉、水仙花、青花菜、地被菊、苹果、梅花、番茄、石斛、毛桃、苎麻、慈姑、葛根、甘肃桃、玫瑰花、槟榔果、甜糖菊、硅藻、牡丹、胡杨、油桐果、梨子皮、板栗花序、青皮云杉、红树根、铁线蕨、黄瓜、小麦叶子种子、番木瓜、甘薯、紫薯、油松、油茶、马尾松、芜菁、毛果杨、木薯、大叶落地生根、山杏、旱柳、桉树、琵琶花果、丹参、人参、西洋参、栀子、洋葱、红豆杉、梨树叶、五倍子、泡桐、西瓜、芍药、雪莲等等,其中包括qiagen无法提取的黑加仑、冬青、月季、松针、葡萄叶片等,promega无法提取的海棠等样品、均可用该产品成功提取。

真菌:桃褐腐病菌(Monilinia fructicola)、菇类等。

推荐:北京艾德莱生物的EASYspin 植物RNA提取试剂盒为独家产品,世界领先水平,不使用苯酚,氯仿有毒性物质,采用离心柱型操作,添加有自主创新的多糖多酚去除成分,是多糖多酚样品的特效产品,20分钟内得到高质量RNA。成功提取Promega无法提取的海棠叶片、Qiagen无法提取的黑加仑。是国产试剂赶超世界先进水平的一个典型例子。

 使用该产品发表的部分文章:http://www.hauserandhauser.com/news-show.asp?id=141
 产品宣传页:http://www.hauserandhauser.com/news-show2.asp?id=102

 

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